Combining a Mutant Allele of FAD2-1A with HD Improves the ω-6/ω-3 Ratio in Soybeans

The intake of foods with unbalanced ω-6/ω-3 ratios causes various health problems. Commodity soybeans generally have a ratio 6–7:1. recommended in terms benefits is <4:1. This study aimed to identify the appropriate combination mutant alleles that can reduce using three segregating soybean populations. F2 individuals from each population were genotyped for different microsomal delta-12 fatty acid desaturase 2 enzyme (FAD2-1A) and an allele homeodomain-like transcriptional regulator (HD) genes, their five major acids assessed. seeds carrying both fad2-1a hd had slightly according alleles. fad2-1aDEL, fad2-1aS117N, fad2-1aW293STOP combined resulted range 1.9–2.7:1, 2.7–3.9:1, 2.6–3.6:1 seeds, respectively. revealed induction mutations FAD2-1ADEL HD was most efficient strategy improve elevate ω-3 concentrations seeds. These results provide useful information breeding programs release new cultivar lower elevated acids, which be beneficial ingredient soybean-based foods.